Alicorns

Alicorns, referred to as Princess Ponies in Hasbro's toy line, and, once, as Pegasus unicorns on Hasbro's My Little Pony Facebook page, are pony characters in the show who have both a unicorn horn and Pegasus wings, who are all styled as royalty. Meghan McCarthy, the show's head writer, says that the title of "princess" is one that is earned in Equestria.

The third season episode Magic Duel marks the first use of the term "alicorn" in the series, and in Magical Mystery Cure the term is used for the first time to directly refer to winged unicorns.

The regal sisters
Princess Celestia and her sister Princess Luna are introduced in the first episode in a series of medieval-like drawings with a narration that says there were "two regal sisters who ruled together and created harmony for all the land," and that "the elder used her unicorn powers to raise the sun at dawn. The younger brought out the moon to begin the night." Lauren Faust commented that Princess Celestia was originally supposed to be Queen Celestia, the highest authority with no parents who outrank her. Her status was later changed to "princess" at Hasbro's request. Faust has stated that Celestia embodies the traits of all three pony kinds: Earth, Pegasus, and unicorn. In Enterplay's trading card series, Series 1 lists the sisters' pony type as both Pegasus and unicorn but not Earth, and Series 2 lists their pony type as all three and refers to them as "immortal alicorns"; a writer and consultant for both series has stated that the inclusion of Earth was "an intentional improvement" they made on Series 2, "based on feedback and it being one of those 'could be canon' sorts of things that just plain makes sense." Faust has suggested that there is more to Princess Celestia than has been depicted of her in the series, and that she wanted Celestia to be "mysterious and hard to understand".

Princess Cadance
The second-to-last episode of the second season introduces Princess Cadance. She is Twilight Sparkle's foal-sitter in Twilight's flashback, and she becomes her sister-in-law after marrying Shining Armor in A Canterlot Wedding - Part 2. Various promotional materials, including a New York Times ad, A Canterlot Wedding - Part 1's Hubworld, iTunes, and Netflix summaries, the back cover of the DVD Royal Pony Wedding, and the home page of Treehouse TV's Royal Wedding Contest site, state that she is the niece of Princess Celestia. A blurb on one of the toy sets featuring Cadance says that she and Shining Armor "lead the Crystal Empire together!" Indeed, a background pony proclaimed her as "The Crystal Princess!" at the end of The Crystal Empire - Part 2. In Enterplay's trading card series, Series 1 lists her pony type as both Pegasus and unicorn but not Earth, and Series 2 lists her pony type as all three; a writer and consultant for both series has stated that the inclusion of Earth was "an intentional improvement" they made on Series 2, "based on feedback and it being one of those 'could be canon' sorts of things that just plain makes sense."

The book Twilight Sparkle and the Crystal Heart Spell states that Cadance was born a Pegasus, and became an Alicorn later.

Queen Chrysalis
Queen Chrysalis was described in her debut episode's script as "a GANGLY BLACK PEGACORN with a gnarled black horn." She and the other changelings were designed by Rebecca Dart, the main character designer for season two. Rebecca was inspired by the work of manga artists Junko Mizuno and Hideshi Hino, and after reading the name "Chrysalis", she "figured [Chrysalis] should be insect-like." The changelings are referred to as a different species in the show and not as ponies.

Twilight Sparkle
Twilight Sparkle is introduced in the show as a unicorn, and she even calls herself a "regular old unicorn" in A Canterlot Wedding - Part 1, to which Princess Cadance replies "You are anything but a regular old unicorn." According to Meghan, "princess [in Equestria] is a designation that’s earned, not freely given and though princesses have specific leadership roles in pony society, being one really means 'being a good pony who shares the gifts that they have been given with others.'" Twilight becomes a princess and gains wings in the episode Magical Mystery Cure.

Twilight Sparkle and the Crystal Heart Spell
The book Twilight Sparkle and the Crystal Heart Spell describes Twilight's new status in chapter 1, "A Crown Achievement": "Twilight had received her very own set of wings. Real Pegasus wings! She was officially a part of a special breed of pony called an Alicorn. This meant that Twilight was now able to harness the magical powers of the Unicorns, the flight abilities of the Pegasi, and the strength of a good, true heart of an Earth Pony."

Other winged unicorns
The show includes other Alicorns and a crew member of the show has explained them as animation errors. These include a filly in Twilight's imagination in Lesson Zero having both wings and a horn and three royal guards in one of Twilight's flashbacks in A Canterlot Wedding - Part 1 having both wings and horns.

There is an illustration that shows a Alicorn princess mare and an Earth pony prince stallion in the book that the Cutie Mark Crusaders read in Hearts and Hooves Day.

Pinkie Pie briefly dresses up as an Alicorn in the episode Magical Mystery Cure. In The Mysterious Mare Do Well, the masked Mare Do Well is shown to have a unicorn horn and Pegasus wings, convincing Rainbow Dash that she's an Alicorn. However, it should be noted that she is never seen with both her horn and wings at the same time.

In the episode Sonic Rainboom, Rarity is given wings by a magic spell temporarily, made of gossamer and morning dew. These wings are similar to those of the flutter ponies from Generation 1. The Glimmer Wings toys include a similarly butterfly-winged Rarity, packaged with a butterfly; according to this release's descriptions, Rarity "imagines having beautiful wings just like her butterfly friend". In Gameloft's mobile game, magic wings of the same design as in Sonic Rainboom are temporarily given to any non-Pegasus for the flying minigame, including the unicorns DJ Pon-3, Fancy Pants, Flam, Flim, Forsythia, Joe, Lovestruck, Lyra Heartstrings, Minuette, Prince Blueblood, Rarity, Shining Armor, Snails, Snips, Sweetie Belle, Trixie, Twilight Sparkle, Twilight Velvet, Twilight's Dad, Twinkleshine, Unicorn Guard, and Upper Crust. WeLoveFine's merchandise designs "Rarity Fabulous Bag" and "Flight of the Ponies" also depict Rarity with wings of gossamer and morning dew.

An Alicorn called Princess Skyla is included in the So Soft Newborn toys.

The player-designed ponies in Adventures in Ponyville, who can only be mares, can be Earth ponies, Pegasi, or unicorns, and for the Rainbow Dash's Cloud Bump minigame, Twilight Sparkle uses magic to give a player-designed pony and an unnamed Earth pony each a set of Pegasus wings temporarily; thus, if the player-designed pony is a unicorn, she will be an Alicorn for the minigame. The player-designed ponies in PonyMaker and its Rarity's Bridal Boutique version, who can be of any sex, can be Earth ponies, Pegasi, unicorns, or Alicorns.

Gameloft's mobile game includes Fleur Dis Lee as an Alicorn, described as a unicorn. A model sheet for the game includes "Sweetiebelle [sic]" as an Alicorn.

In a French My Little Pony magazine story, Celestia has a cousin named Léon, a four-year-old Alicorn.

WeLoveFine's merchandise designs have included several Alicorn depictions. "Dr. Hooves Line", "Dr. Hooves", "Hooves Scribble", and "Dr [sic] Hooves" include alicorn mares similar to Weeping Angels from Doctor Who; "Grown Up Ponies" included Fluttershy and Rainbow Dash both as Alicorns; "Engineered Alicorn" and "Steampunk Trixie" respectively included and include Twilight Sparkle and Trixie each equipped with steampunk wings; "All Dem Ponies" included six Alicorns, three of them mares and three of them stallions, with one of the stallions resembling Discord; "Iconic Print" included an Alicorn mare who was originally created by Lauren Faust; and "We're All Doomed" includes Derpy as an Alicorn.

The Alicorn Amulet
The Alicorn Amulet is an amulet which features the image of a winged unicorn. Trixie uses it to become a "highest level unicorn" and gain immense magical powers and antagonize the citizens of Ponyville in the episode Magic Duel. Twilight Sparkle finds out that only the wearer can remove the amulet and tricks Trixie into removing it with a fake amulet of her own an a magic duel.