Owlowiscious

Owloysius is an owl that first appears in Owl's Well That Ends Well and gets adopted by Twilight Sparkle. He initially raises Spike's suspicions, but eventually helps Twilight in rescuing Spike from another dragon. He appears again in the episode May the Best Pet Win!

Character design and personality
Owloysius' character design stands out from other males in the series for having pronounced eyelashes, while all other males do not. He is intelligent enough to understand and perform Twilight's various requests, such as retrieving specific books from bookshelves, though he only hoots and never speaks. He accompanies Twilight throughout the episode, and helps find and rescue Spike from a fully grown dragon, taking great risks in the process.

Owl's Well That Ends Well
Owloysius first appears when he retrieves one of Twilight Sparkle's papers, which had blown away in the wind. Twilight notes the cold weather outside and invites the owl to spend the night in her house. She spends a good portion of the night working on her paper about comets, with Owloysius assisting. The next morning, Spike finds out that Twilight had decided to take Owloysius as a "junior assistant", to lessen Spike's workload, since she noticed that he regularly works himself to the point of exhaustion.

Spike is quite jealous of Owloysius, outwardly proclaiming that he suspects Owloysius is trying to replace him as Twilight's assistant. His worries only deepen when Twilight rebukes him after Owloysius discovers a book that Spike had accidentally burned by sneezing on it and hidden in a bookshelf. Fearing for his position as Twilight's assistant, Spike attempts to frame Owloysius for killing a mouse using ketchup and one of Opalescence's toy mice in hopes that Twilight would be angry with Owloysius and get rid of him. However, Twilight and Owloysius walk in on Spike while he's still preparing the "crime scene", and Twilight expresses disappointment in Spike's actions. Distraught, Spike decides to run away to the Everfree Forest.

Later that night, Owloysius helps Twilight find and rescue Spike from a fully-grown dragon, whom Spike had enraged by intruding in his cave and eating his gemstones. Owloysius gives Spike an opportunity to escape by distracting the dragon. Once Spike is out of the cave, Owloysius once again helps out Twilight and Spike by guiding them through the dark and obstructed path through the forest. Once they reach safety, Twilight explains that Owloysius was able to track Spike down by following his footprints, which were stained with ketchup from Spike's earlier framing attempt. In the end, Spike realizes with Twilight's help that Owloysius was never trying to replace him, but instead served as her nighttime assistant while he was asleep. Spike apologizes for his actions to the two, with an apology in return from Twilight for not realizing how sensitive Spike can be.

May the Best Pet Win
Owloysius appears again in May the Best Pet Win!, first in Rainbow Dash's nightmare and then at the Pony Pet Playdate along with the other ponies' pets. He tags along with Twilight Sparkle through most of the episode.

Merchandise
One Hasbro toy of Twilight Sparkle comes with an owl figurine. This owl, however, is not Owloysius, as it is pink and has a different body shape.

Gallery

 * Owloysius image gallery