Humans

Humans have been alluded to on several occasions: Groucho glasses with human noses on them appear in three episodes; Party of One, where Pinkie Pie and Gummy each wear a pair of Groucho glasses as part of their disguise; It's About Time, where Pinkie's party supplies include multiple pairs of Groucho glasses, one of which is inadvertently worn by Twilight Sparkle after the unicorn crashes into Fluttershy carrying the supplies; and Magical Mystery Cure, where Fluttershy, Spike, and Pinkie wear Groucho glasses. In Sonic Rainboom, Pinkie Pie wears foam finger which represents a human hand while cheering for Rainbow Dash's performance. A drawing of a human silhouette pinned to the wall appears in episodes Baby Cakes and A Friend in Deed. Iron Will, who appears in the episode Putting Your Hoof Down, is a minotaur: a therianthropic hybrid of a human and a bull. Creatures such as dragons, Diamond Dogs, draconequi and griffons have opposable thumbs and can talk.

Various humans have appeared along with ponies in merchandise and in other media such as commercials, some humans being cartoon characters and others being played by live-action actors. Cartoon humans include characters who appear on some of WeLoveFine's My Little Pony Project 2012 art shirts, characters who appear in Electronic Arts' Hasbro iOS app Monopoly Hotels, and characters from other Hub-aired shows who appear in some of the channel's promotional crossover media. Live-action humans include the self-played Tori Spelling in nine Hub promos set around A Canterlot Wedding - Part 1 and A Canterlot Wedding - Part 2, the self-played Mallory Hagan in Hub promos set around Magical Mystery Cure, young girls in other promos (with Princess Celestia in one for Target's Canterlot toys and other merchandise, with Princess Cadance in one for the Pony Wedding talking toy of her, and/or with Pinkie Pie and Rarity in ones for the Pony Wedding toy of the Pony Princess Wedding Castle), students and a teacher at Hub High School with Rainbow Dash and Megatron from Transformers Prime in the Hub promo Hub High, and members of The Aquabats in The Aquabats! Super Show! episode Cowboy Android! and in some of The Hub's promotional crossover media. The UK &amp; Ireland version of Boomerang's promotional webpage and commercial for their 2011 Make it a My Little Pony Christmas competition both show Santa in silhouette, with his flying sleigh pulled by Fluttershy and Twilight Sparkle on the webpage and by Princess Celestia in the commercial. Santa Claus was similarly an unseen character in previous generations of My Little Pony.

On August 28, 2011, Jayson Thiessen was asked regarding the show "Humans? Please tell me that you will not add them at any point" and answered "heavens no!" On August 5 and October 6, 2012, comic book artist Andy Price respectively stated regarding the comic "Humans: Nope. Nothing that does a major stray from what is established in the show" and "WILL THERE BE HUMANS? No. We will not be changing the direction set by the show, nor will we introduce elements that do not fit the show."

In My Little Pony Equestria Girls, Twilight Sparkle is transformed into a human after she goes to a world populated by humans with multicolored skin, and while there, she meets or sees a few humans who strongly resemble certain ponies in Equestria.