Talk:Episodes, films, and shorts/@comment-1031724-20160827021823/@comment-1031724-20160827164229

In order they were introduced, it's Twilight, Applejack, Rainbow Dash, Rarity, Fluttershy, and Pinkie Pie (though if you count appearances in general, Pinkie comes before Applejack), and in the opening sequence, it's Twilight, Rainbow, Pinkie, Rarity, Applejack, and Fluttershy